Folate Receptor-Alpha Targeted 7x19 CAR-γδT Suppressed Triple-Negative Breast Cancer Xenograft Model in Mice

Figure 1

γδ T cells were successfully separated and expanded from healthy donor PBMCs and transduced with CAR structure. (a) Schematic representation of 7 × 19 CAR and conventional CAR constructs. (b) The cells were detected by flow cytometry and representative dot plot from the same donor showing the percentage of Vγ9Vδ2 cells in PBMCs (left) and after the MACS magnetic beads sorting (right). (C) FACS analysis of sorting γδT cells transduction efficiency. The sorted γδT were stimulated with ZOL, IL-2, and IL-21 and transfected with either 7 × 19 CAR (middle) or conventional CAR (right). The untransduced γδT were treated as negative control.

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