Folate Receptor-Alpha Targeted 7x19 CAR-γδT Suppressed Triple-Negative Breast Cancer Xenograft Model in Mice

Figure 2

Chemotactic capacity towards γδT, CAR-γδT, and 7 × 19 CAR-γδ T cells. (a) FACS analysis of the expression of CCR7 in human dendritic cells (left), PBMCs (middle), and αβ T cells (right). (b) IL-7/CCL19 secretion of γδT, CAR-γδT, and 7 × 19 CAR-γδT were detected by ELISA, respectively ( ＜ 0.01). (c) The purified αβ T cell migrated from the upper chamber to the lower chamber and was analyzed by flow cytometry ( ＜ 0.01). (d) B cells and CFSE-labeled dendritic cells migration driven by CXCL13 and CCL19 were counted using flow cytometry. P values were calculated by Student’s two-sided t-test.

(a)

(b)

(c)

(d)