LINC01535 Attenuates ccRCC Progression through Regulation of the miR-146b-5p/TRIM2 Axis and Inactivation of the PI3K/Akt Pathway

Figure 6

LINC01535/miR-146b-5p/TRIM2 axis inhibits PI3K/Akt activation in ccRCC progression. The protein levels of p-PI3K, PI3K, p-Akt (T308 and S473), and Akt in ccRCC cells (a, b) were measured by western blot after different treatment. Caki-1 and 786-O cells were transfected with miR-146b-5p inhibitor or pcDNA3.1-TRIM2 and then treated with 740Y-P (20 μmol/L), followed by detection of proliferation (c, d) by CCK-8 assays and invasion (e, f) by transwell assays. .

(a)

(b)

(c)

(d)

(e)

(f)