Overexpression of PTPRZ1 Regulates p120/-Catenin Phosphorylation to Promote Carcinogenesis of Oral Submucous Fibrosis

Figure 2

Overexpression of PTPRZ1 regulated the phosphorylation of p120/-catenin. (a, b) qRT-PCR and western blot were used to detect PTPRZ1 expression in each group of cells (n = 3). vs. control group (HNOEC cells). (c–e) qRT-PCR was performed to detect the expression of PTPRZ1, p120, and -catenin in each group of cells (n = 3). (f) Western blot detected PTPRZ1, p120, and p--catenin/-catenin protein expression in each group of cells (n = 3). (g) Western blot detected p-120 protein expression of cytoplasm or nucleus in each group of cells (n = 3). (h) IF detected the distribution of p-p120 and p-β-catenin in the oe-NC and oe-PTPRZ1 groups (hOMF cells, n = 3). vs. the OSF group (hOMF cells); ^#P < 0.05 vs. the oe-NC group (hOMF cells); scale bar = 25 µm; and the magnification is 400 times.

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