Overexpression of PTPRZ1 Regulates p120/-Catenin Phosphorylation to Promote Carcinogenesis of Oral Submucous Fibrosis

Figure 4

Knockdown of p120 pathway promoted oral cell carcinogenesis. (a) qRT-PCR was used to detect p120 expression in each group of cells. vs. the sh-NC group (hOMF cells, n = 3). (b) CCK-8 detected the proliferation rate of cells in each group at 12 h, 24 h, 48 h, 72 h, 96 h, and 120 h (n = 3). (c) qRT-PCR detected p120, RhoA, Rac1, and CDC42 expressions (n = 3). (d) Western blot detected p-p120, p120, RhoA, Rac1, and CDC42 protein expression levels (n = 3). (e) EDU was performed to detect the proliferation rate of cells (n = 3). (f) Flow cytometry detected cell cycle in each group (n = 3). (g) Annexin V-FITC/PI flow cytometry was applied to detect the apoptosis rate of cells in each group (n = 3). vs. sh-NC group; scale bar = 100 µm; and the magnification is 100 times.

(a)

(b)

(c)

(d)

(e)

(f)

(g)