Overexpression of PTPRZ1 Regulates p120/-Catenin Phosphorylation to Promote Carcinogenesis of Oral Submucous Fibrosis

Figure 5

Activated -catenin pathway promoted oral cell carcinogenesis. (a) CCK-8 detected the proliferation rate of cells in each group at 12 h, 24 h, 48 h, 72 h, 96 h, and 120 h (n = 3). (b) qRT-PCR was used to detect -catenin, cyclin D1, and c-myc expressions (n = 3). (c) Western blot was used to detect -catenin, p-β-catenin, cyclin D1, and c-myc protein expression levels (n = 3). (d) EDU detected the proliferation rate of cells in each group (n = 3). (e) Flow cytometry was performed to detect cell cycle (n = 3). (f) Annexin V-FITC/PI flow cytometry was used to detect the apoptosis rate of cells (n = 3). vs. the oe-NC group (hOMF cells); scale bar = 100 µm; and the magnification is 100 times.

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