MiR-526b-3p Inhibits the Resistance of Glioma Cells to Adriamycin by Targeting MAPRE1

Figure 4

MAPRE1 is a downstream target gene of miR-526b-3p. (a) Prediction of the target genes of miR-526b-3p and RT-qPCR detected these genes expression in normal and glioma cells. (b) The putative target sequence of miR-526b-3p in MAPRE1 3′UTR and mutated sequence. (c) Luciferase signal was detected in U87 and U251 cells transfected with wild-type or mutant MAPRE1, and miR-526b-3p mimics or NC mimics. (d) Pull-down assay detected the miR-526b-3p target binding the MAPRE1 in U87 and U251 cells. (e) RT-qPCR and western blot detected the MAPRE1 expression in U87 and U251 cells that transfected miR-526b-3p mimics or NC mimics. (f) RT-qPCR detected MAPRE1 expression in glioma and adjacent normal tissues. (g) RT-qPCR detected MAPRE1 expression in ADR nonresistant and resistant glioma tissues. (h) The expression correlation between miR-526b-3p and MAPRE1 in glioma tissues. (i) RT-qPCR detected MAPRE1 expression in glioma cells. ^{∗∗∗∗∗∗}. ^∗indicates the difference with the control group. Error bars were represented the mean ± SD of triplicate experiments.

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