MiR-526b-3p Inhibits the Resistance of Glioma Cells to Adriamycin by Targeting MAPRE1

Figure 5

MiR-526b-3p inhibits the development and ADR resistance of glioma through MAPRE1. U87 and U251 cells transfected MAPRE1 vector or control vector, respectively. The efficiency of MAPRE1 overexpression was detected by RT-qPCR. (a) U87 and U251 cell transfected miR-526b-3p mimics and NC vector or transfected miR-526b-3p mimics and MAPRE1 vector simultaneously. Transfect NC mimics and control vector group cells were used as the negative control. (b) Cell viability was detected by CCK8 assay. (c) Colony formation assay detected cell proliferation. (d) Flow cytometry detected cell apoptosis ratio. (e) Cell migration and cell invasion were detected through a Transwell assay. (f) RT-qPCR detected the expression of MAPRE1 in ADR nonresistant and ADR-resistant U87 and U251 cells. (g) The expression of MAPRE1 in U87/ADR and U251/ADR cells that transfected the MAPRE1 vector or control vector. U87/ADR and U251/ADR cells transfected miR-526b-3p mimics and NC vector or miR-526b-3p mimics and MAPRE1 vector simultaneously. Cell inhibition ratio (h), cell apoptosis ratio (i), cell migration, and cell invasion (j) were detected in transfected ADR-resistant cells. ^{∗∗∗∗∗∗}vs the NC mimics and control vector cotransfected cells. ^###, ^## P, ^# vs. the miR-526b-3p mimics and control vector cotransfected cells. Error bars were represented the mean ± SD of triplicate experiments.

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