Long Noncoding RNA SNHG16 Regulates the Growth of Human Lung Cancer Cells by Modulating the Expression of Aldehyde Dehydrogenase 2 (ALDH2)

Figure 2

SNHG16 repression reduced the viability of lung cancer cells through apoptosis. (a) Confirmation of SNHG16 transcriptional knockdown in A549 and SK-LU-1 lung cancer cells, (b) determination of viabilities of A549 and SK-LU-1 lung cancer cells transfected with si-NC or si-SNHG16 by MTT assay, (c) colony formation assay showing colony formation of A549 and SK-LU-1 cells transfected with si-NC or si-SNHG16, (d) DAPI staining of A549 and SK-LU-1 lung cancer cells transected with si-SNHG16 or si-NC (arrows depict apoptotic cells), (e) flow cytometric analysis for detection of apoptosis of A549 and SK-LU-1 lung cancer cells transected with si-SNHG16 or si-NC, and (f) western blotting of Bax and Bcl-2 marker proteins form A549 and SK-LU-1 lung cancer cells transected with si-SNHG16 or si-NC. Each experiment was performed in triplicates, and difference between two values was considered significant at .

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