Long Noncoding RNA SNHG16 Regulates the Growth of Human Lung Cancer Cells by Modulating the Expression of Aldehyde Dehydrogenase 2 (ALDH2)

Figure 4

SNHG16 interacted and repressed ALDH2 in lung cancer, posttranscriptionally. (a) In silico analysis for prediction of SNHG16 and ALDH2 interaction in lung cancer through LncRRIsearch software; (b) in silico analysis for prediction of SNHG16 and ALDH2 interaction in lung cancer through VARNA: Visualization Applet for RNA software; (c) dual luciferase assay showing interaction between SNHG16 and ALDH2; (d) relative gene expression of ALDH2 in normal and cancerous lung tissues; (e) IHC analysis showing expression of ALDH2 in three pairs (I, II, and III) of normal and cancerous tissues; (f) relative gene expression of ALDH2 at different pathological stages of lung cancer; (g) relative gene expression of ALDH2 in normal lung cell line (MRC5) and lung cancer cell lines (A427, A549, HCC827, and SK-LU-1); (h) relative protein expression of ALDH2 in A549 cancer cells transfected with si-SNHG16 or si-NC. Each experiment was performed with three replicates ().

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