Long Noncoding RNA SNHG16 Regulates the Growth of Human Lung Cancer Cells by Modulating the Expression of Aldehyde Dehydrogenase 2 (ALDH2)

Figure 5

ALDH2 modulated the regulatory role of lncRNA-SNHG16 in lung cancer. (a) Estimation of viabilities of A549 cancer cells by MTT assay at different culture stages, transfected with pcDNA-ALDH2 or control vector pcDNA3.1 (NC); (b) colony formation of A549 cancer cells transfected with pcDNA-ALDH2 or control vector pcDNA3.1 (NC); (c) DAPI staining of A549 cancer cells transfected with pcDNA-ALDH2 or NC; (d) flow cytometry for analysis of apoptosis of A549 cancer cells transfected with pcDNA-ALDH2 or NC; (e) estimation of viabilities of A549 cancer cells by MTT assay at different culture stages, transfected with si-SNHG16, pcDNA-ALDH2, or si-SNHG16 plus si-ALDH2; (f) colony formation of A549 cancer cells transfected with si-SNHG16, pcDNA-ALDH2, or si-SNHG16 plus si-ALDH2; (g) DAPI staining of A549 cancer cells transfected with si-SNHG16, si-NC, or si-SNHG16 plus si-ALDH2; (h) flow cytometry for analysis of apoptosis of A549 cancer cells transfected with si-SNHG16, si-NC, or si-SNHG16 plus si-ALDH2. Three replicates were used for performing the experiments () (arrows depict apoptotic cells).

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