circSYPL1 Promotes the Proliferation and Metastasis of Hepatocellular Carcinoma via the Upregulation of EZH2 Expression by Competing with hsa-miR-506-3p

Figure 2

In vitro, silencing circSYPL1 decreases proliferation and metastasis of HCC but enhances apoptosis. (a) qPCR showing the level of circSYPL1 in PLC-PRF-5 cells treated with circSYPL1 or negative control siRNAs. (b) qPCR showing the expression level of circSYPL1 in HepG2 cells treated with circSYPL1 or negative control siRNAs. (c) Cell proliferation assay of PLC-PRF-5 cells treated with circSYPL1 or negative control siRNAs by the BrdU test. (d) Cell proliferation assay of HepG2 cells treated with circSYPL1 or negative control siRNAs by the BrdU test. (e) TUNEL assay detected the apoptosis of cisplatin-induced PLC-PRF-5 cells treated with circSYPL1 or negative control siRNAs. (f) TUNEL assay detected the apoptosis of cisplatin-induced HepG2 cells treated with circSYPL1 or negative control siRNAs. (g) Representative CV staining images (left) as well as statistical analyses (right) of a transwell invasion assay using PLC-PRF-5 cells treated with circSYPL1 or a negative control siRNA. (h) Representative CV (left) in addition to statistical analyses (right) of a transwell invasion assay using HepG2 cells treated with circSYPL1 or a negative control siRNA.

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