circSYPL1 Promotes the Proliferation and Metastasis of Hepatocellular Carcinoma via the Upregulation of EZH2 Expression by Competing with hsa-miR-506-3p

Figure 7

miR-506-3p overexpression or EZH2 knockdown affect cancer progression. (a) qPCR showing the expression levels of EZH2 in PLC-PRF-5 cells treated with negative control plasmid, circSYPL1 overexpressed plasmid, circSYPL1 and miR-506-3p mimics, and circSYPL1 and miR-506-3p shRNA. (b) qPCR showing the expression of EZH2 in HepG2 cells treated with negative control plasmid, circSYPL1 overexpressed plasmid, circSYPL1 and miR-506-3p mimics, and circSYPL1 and miR-506-3p shRNA. (c) PLC-PRF-5 cells treated with a negative control plasmid were analyzed for cell survival, circSYPL1 overexpressed plasmid, circSYPL1 and miR-506-3p mimics, and circSYPL1 and miR-506-3p shRNA by the CCK-8 test. (d) Cell survival assay for HepG2 cells treated with negative control plasmid, circSYPL1 overexpressed plasmid, circSYPL1 and miR-506-3p mimics, and circSYPL1 and miR-506-3p shRNA by the CCK-8 test. (e) Cell invasion assay for PLC-PRF-5 cells treated with negative control plasmid, circSYPL1 overexpressed plasmid, circSYPL1 and miR-506-3p mimics, and circSYPL1 and miR-506-3p shRNA by the transwell test. (f) Cell invasion assay for HepG2 cells treated with negative control plasmid, circSYPL1 overexpressed plasmid, circSYPL1 and miR-506-3p mimics, and circSYPL1 and miR-506-3p shRNA by the transwell test.

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