CircFMN2 Boosts Sorafenib Resistance in Hepatocellular Carcinoma Cells via Upregulating CNBP by Restraining Ubiquitination

Figure 2

CNBP was confirmed as the binding protein of circFMN2. CNBP level was measured via Western blot (a), (b) lanes in panel A were corresponding to columns in panel B, Western blot analysis of CNBP level after RNA pull-down assay (c), circFMN2 level detected by RT-qPCR following RNA immunoprecipitation assay (d), The deletion fragments, sense strand and anti-sense strand of circFMN2 in deletion-mapping analysis (e), Western analysis of CNBP level following RNA pull-down assay with different circFMN2 constructs in deletion-mapping analysis (f), and ubiquitination level detected by Western blot assay (g). vs. control group; ^###, vs. circFMN2-NC group; ^&&&, vs. MDR group; ^$$$, vs. MDR + siRNA-NC group.

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