Digital Image Analysis-Based Evaluation of Claudin-1 and Claudin-7 Delocalization in Cutaneous Squamous Cell Carcinoma and in Its Precancerous State

Figure 1

Immunohistochemical analysis. (a) Subcellular localization of Claudin-1 and Claudin-7 and p-ERK, p-AKT, and p-mTOR expression in sun-exposed skin, precancerous actinic keratosis (AK), in situ Bowen’s disease (BD), and invasive cutaneous squamous cell carcinoma (SCC) (×400). Quantitative analysis of Claudin-1 and Claudin-7 immunostaining for (b) membrane (Mem, IM scores), (c) cytoplasm (Cyto, %), and (d) nucleus (Nuc, %) localization reflected by positive percentages in sun-exposed skin (green), AK (yellow), BD (blue), and SCC (red). A comparison of positive percentages of (e) p-ERK, (f) p-AKT, and (g) p-mTOR. Relative to sun-exposed skin: ; ; . Relative to AK tissue: ^#; ^##; ^###. Relative to BD tissue: ^†; ^††; ^†††.

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