LncRNA UCA1 Promotes the Progression of AML by Upregulating the Expression of CXCR4 and CYP1B1 by Affecting the Stability of METTL14

Figure 3

METTL14 influenced m6A methylation affected by lncRNA UCA1. (a) Dot blot assay was used to detect m6A methylation level in AML patients. (b, c) The expression of METTL14 in AML patients was detected by qRT-PCR and western blot, respectively. β-actin act as control. (d) m6A methylation level affected by UCA1 was detected by dot blot assay. (e) The expression of lncRNA UCA1 affected by suppression of METTL14 was detected by qRT-PCR. (f) Immunofluorescence staining was used to detect the expression of METTL14 affected by UCA1. (g) Colocalization of UCA1 and METTL14 in cytoplasm and nucleus. (h) RNA pull-down followed by western blot was used to detect the binding relationship between UCA1 and METTL14 protein. The data were presented by mean ± SD.  < 0.005 and  < 0.001.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)