The PARP1 Inhibitor Niraparib Represses DNA Damage Repair and Synergizes with Temozolomide for Antimyeloma Effects

Figure 2

Effects of TMZ and/or Nira on proliferation and apoptotic death in MM cells. (a) RPMI8226 and NCI-H929 cells were administered TMZ and/or Nira for 48 h, and apoptosis was examined flow-cytometrically after Annexin V–FITC/PI staining. (b) RPMI8226 and NCI-H929 cells were administered TMZ and/or Nira for 48 h, and S-phase cells were detected flow-cytometrically by the EdU assay. (c, d) Quantification of apoptotic and S-phase cells shown in (a). (e) Cell cycle-related and apoptosis-associated proteins in RPMI8226 and NCI-H929 cells were quantitated by immunoblot. , , and versus control group; ^## and ^### versus TMZ group; ⁺⁺ and ⁺⁺⁺ versus Nira group. TMZ: temozolomide; Nira, niraparib.

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