Hax-1 Regulates Radiation-Induced Mitochondrial-Dependent Apoptosis of Uveal Melanoma Cells through PI3K/AKT/eNOS Pathway

Figure 6

HAX-1 regulates uveal melanoma cell viability, migration, tumor cell spheroidization ability, and mitochondrial-dependent apoptosis by regulating the AKT/eNOS pathway. (a) CCK-8 method was employed to identify the proliferative effect of MUM-2B and C91 cells in siHAX-1+ LY294002 and siNC+LY294002 groups. siHAX-1 vs. the controls, and ; siHAX-1+LY294002 vs. siNC+LY294002, and . (b) Scratch test to evaluate the effect of HAX-1+LY294002 on the migration of MUM-2B and C91 cells. (c) The clone formation experiment detects the effect of siHAX-1+LY294002 on the proliferation of MUM-2B and C91 cells. siHAX-1 vs. the controls, and ; siHAX-1+LY294002 vs. siNC+LY294002, and . (d) Migration test (using Matrigel Transwell chambers) is used to study cell migration. siHAX-1 vs. control group, and ; siHAX-1+LY294002 vs. siNC+LY294002, and . (e) Tumor spherule forming ability experiment to assess the role of siHAX-1+LY294002 in the tumor spherule forming capability of MUM-2B and C91 cells. siHAX-1 vs. the controls, and ; siHAX-1+LY294002 vs. siNC+LY294002, and . (f) Flow cytometry experiment to assess the roles of siHAX-1+LY294002 in the apoptotic capability of MUM-2B and C91 cells. siHAX-1 vs. the controls, and ; siHAX-1+LY294002 vs. siNC+LY294002, and .

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