Effects of the Targeted Regulation of CCRK by miR-335-5p on the Proliferation and Tumorigenicity of Human Renal Carcinoma Cells

Figure 2

CCRK promoted ccRCC cell proliferation in vitro. (a) and (b) Analysis of the CCRK expression and cell proliferation rates in A498 and ACHN cells transfected with scr-shRNA or CCRK-shRNA by western blotting and CCK-8 assay. (c) The CCRK plasmid (pcDNA3.1) was transfected by Lipofectamine 2000 in A498 cells with knockdown of CCRK, CCRK expression and cell proliferation were partly recovered. (d) Colony formation assay in A498 and ACHN cells.

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