Effects of the Targeted Regulation of CCRK by miR-335-5p on the Proliferation and Tumorigenicity of Human Renal Carcinoma Cells

Figure 4

Western blotting in vitro and tumour volume analysis in vivo. (a) Analysis of the expression of cell cycle marker (cyclin D1) and apoptosis markers (caspase-3, cleaved caspase-3, and Bax) in A498 and ACHN cells transfected with or without lentivirus CCRK-shRNA by western blotting. (b) Analysis of tumour volume in vivo after subcutaneous inoculation of nude mice in A498 cells transfected with or without lentivirus CCRK-shRNA (n = 4 mice). (c) The IHC stain in tumour tissues of nude mice (n = 3). (d) Analysis of optical density (OD) value of Ki-67 index in tumour tissues of nude mice (n = 3).

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