Effects of the Targeted Regulation of CCRK by miR-335-5p on the Proliferation and Tumorigenicity of Human Renal Carcinoma Cells

Figure 5

miR-335-5p binding to 3’UTR of CCRK and its effects on ccRCC patients. (a) The volcano plot shows the upregulated (red dot) and downregulated (light-green dot) miRNAs in ccRCC patients and normal patients. (b) The Venn diagram showed the overlapping miRNAs (yellow, 5) between predicted CCRK-targeting miRNAs (blue, 422) by TargetScan 3.1 and downregulated miRNAs (orange, 15) in ccRCC tissues. (c) Comparison of the miR-335-5p expression levels between normal tissues and primary tumour tissues (ccRCC) based on TCGA database. (d) The relative expression of miR-335-5p in RCC cells (A498, 786-O, Caki-1, and ACHN). (e) Comparison of the relative expression of miR-335-5p among tumours of different pathological grades; (f) The miR-335-5p and CCRK 3′UTR had conservative binding sites. (g) The expression of CCRK protein whether adding miR-335-5p mimic into A498 and ACHN cells. (h) Analysis of the luciferase activity between miR-335-5p group and miR-NC group in A498 and ACHN cells transfected with wild-type (wt) or mutant (mu) CCRK 3′UTR. ; ; and .

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