Research Article
Effects of the Targeted Regulation of CCRK by miR-335-5p on the Proliferation and Tumorigenicity of Human Renal Carcinoma Cells
Figure 6
expression of CCRK attenuated the effects of miR-335-5p in ccRCC cell lines. A498 cells and ACHN cells were transfected with miR-335-5p mimic or miR-NC and cotransfected with miR-335-5p mimic + LV-CCRK or miR-335-5p mimic + LV-NC. (a) and (b) Analysis of the cell proliferation rate of 4 groups in A498 cells and ACHN cells by the CCK-8 assay. (c), (d), (e), and (f) Analysis of the apoptosis rates and proliferation index of 4 groups in A498 cells and ACHN cells by flow cytometry. (g), (h), and (i) Analysis of the expression of CCRK, apoptosis markers (caspase-3, cleaved caspase-3, and Bax), and cell cycle marker (cyclin D1) of 4 groups by western blotting. The data are presented as the mean ± SEM. NC, negative control; LV, lentivirus. ; ; and . compared with miR NC, # compared with miR-335 mimic + LV-NC.
(a) |
(b) |
(c) |
(d) |
(e) |
(f) |
(g) |
(h) |