The Biocomplex Assembled from Antigen Peptide and Toll-like Receptor Agonist Improved the Immunity against Pancreatic Adenocarcinoma In Vivo

Figure 2

In vitro characterization of the biocomplex to principal immune cells, that is, macrophage and DC. (a) Uptake of the biocomplex by macrophage and DC as characterized by flow cytometry. The biocomplex was diluted by different ratios to test the internalization rate at different concentrations. (b) Fluorescence intensity characterizing the internalization of 1× biocomplex. (c) Impact of biocomplex, as well as its component, to the viability of the immune cells. (d) The effect of different biocomplex with different N :  ratios (1 : 5 and 5 : 1) to macrophage and DC viability. (e) The uptake of the biocomplex with different N :  ratios by macrophages and DCs. CTRL : Control (treated with PBS).

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