Deficiency of kin17 Facilitates Apoptosis of Cervical Cancer Cells by Modulating Caspase 3, PARP, and Bcl-2 Family Proteins

Figure 1

Establishment and determination of cervical cells line HeLa and SiHa with kin17 knockdown. (a) morphological features and fluorescence-indicated infection of HeLa^Mock, HeLa^NC, HeLa^KD cells, SiHa^Mock, SiHa^NC, and SiHa^KD cells, ×100. Scale bars = 200 μm. (b) protein levels of kin17 in HeLa cells and SiHa cells transfected with lentivirus were identified by western blotting, and the densitometric quantification was showed. HeLa^KD, HeLa cells transfected with recombinant lentiviral vectors carrying the siRNA-targeting KIN17 gene; HeLa^NC, HeLa cells were transfected with the control vector; HeLa^Mock, HeLa cells without transfection of the vector. SiHa^KD, SiHa cells infected with recombinant lentiviral vectors carrying the siRNA-targeting KIN17 gene; SiHa^NC, SiHa cells transfected with the controlled vector; SiHa^Mock, SiHa cells without transfection of the vector. NC, negative control; KD, knockdown; , n = 3.

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