Biochanin A Suppresses Tumor Progression and PD-L1 Expression via Inhibiting ZEB1 Expression in Colorectal Cancer

Figure 3

The regulatory effect of ZEB1 on PD-L1 expression in CRC. (A, B) Transcriptional and protein expressions of ZEB1 in CRC cells with ZEB1 silencing were examined by qPCR and Western blotting. (C, D) Protein expression of PD-L1 in CRC cells with ZEB1 silencing was examined by immunofluorescence. (E, F) Transcriptional and protein expressions of ZEB1 in CRC cells with ZEB1 overexpression were examined by qPCR and Western blotting. (G, H) Protein expression of PD-L1 in CRC cells with ZEB1 overexpression was examined by immunofluorescence.