Biochanin A Suppresses Tumor Progression and PD-L1 Expression via Inhibiting ZEB1 Expression in Colorectal Cancer

Figure 4

The effect of Biochanin A on ZEB1 expression and EMT in CRC. (A, B) Transcriptional and protein expressions of ZEB1 in CRC cells treated with Biochanin A were examined by qPCR and Western blotting. (C, D) Protein expression of ZEB1 in CRC cells treated with Biochanin A was examined by immunofluorescence. (E, G) Protein expression of E-cadherin and N-cadherin in CRC cells treated with Biochanin A was examined by immunofluorescence. (F, H) Protein expression of E-cadherin and N-cadherin in CRC cells treated with Biochanin A was examined by Western blotting.