Biochanin A Suppresses Tumor Progression and PD-L1 Expression via Inhibiting ZEB1 Expression in Colorectal Cancer

Figure 5

Tumor-suppressive role of Biochanin A in CRC in vitro and in vivo. (a) The proliferative capacity of control and Biochanin A-treated CRC cells was examined by CCK-8 assay. (b) The migratory capacity of control and Biochanin A-treated CRC cells was examined by wound healing assay. (c) Quantitative analysis of cell migratory capacity. (d) The invasive capacity of control and Biochanin A-treated CRC cells was examined by Boyden chamber assay. (e) Quantitative analysis of cell invasive capacity. (f) Tumor volume of the mouse models, which was monitored every three days. (g) Isolated tumor images from these two groups on day 21 after the first treatment. (h) The tumor weight of these two groups; mice were sacrificed on day 21 after the first treatment.