Biochanin A Suppresses Tumor Progression and PD-L1 Expression via Inhibiting ZEB1 Expression in Colorectal Cancer

Figure 6

The effect of Biochanin A on PD-L1 expression in CRC. (A, B) Transcriptional and protein expressions of ZEB1 in CRC cells treated with Biochanin A or transfected with ZEB1 vector were examined by qPCR and Western blotting. (C, D) Transcriptional and protein expressions of PD-L1 in CRC cells treated with Biochanin A or transfected with ZEB1 vector were examined by qPCR and Western blotting. (E, F) Protein expression of PD-L1 in CRC cells treated with Biochanin A or transfected with ZEB1 vector was examined by immunofluorescence. (G) Representative images uncovering ZEB1 and PD-L1 expression in vehicle and Biochanin A treated groups. (H) Semiquantitative analysis of ZEB1 and PD-L1 in mouse tumor tissues. (I) Schematic diagram of the mechanism underlying Biochanin A-induced ZEB1-mediated PD-L1 downregulation.