Artemin Promotes the Migration and Invasion of Cervical Cancer Cells through AKT/mTORC1 Signaling

Figure 2

ARTN promotes proliferation of CC cells in vitro. (a) Western blot analysis showing that HeLa and SiHa cells have ARTN protein (). (b) In HeLa and SiHa cells that had been transfected with the lentivirus, real-time PCR demonstrated the relative ARTN mRNA expression levels (, ). (c)-(d) Western blot analysis revealed the ARTN protein level in HeLa and SiHa cells following transfection with produced lentivirus (, ). (e)-(f) The CCK-8 assays performed to detect the effects of ARTN overexpression and knockdown on the proliferation of HeLa and SiHa cells (, ). (g)-(h) Colony formation assays conducted following ARTN overexpression and knockdown in HeLa and SiHa cells (, ). The colon counts from three independent experiments are represented by error bars.

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