Artemin Promotes the Migration and Invasion of Cervical Cancer Cells through AKT/mTORC1 Signaling

Figure 3

AKT/mTOR-mediated EMT is activated by ARTN, which then encourages CC cell migration and invasion. (a and b) The effects of the treatments on cell migration detected using wound-healing migration assays. The colon counts from three separate studies are represented by error bars (). (c and d) Transwell assays used to determine the invasion abilities. The colon counts from three separate studies are represented by error bars (scale bar, 200 μM, ). (e) IHC staining showing that CC tissues with ARTN and paracancerous tissues had relative P-AKT and P-mTOR protein levels (original magnification, ×400). (f) After ARTN was overexpressed and knocked down in HeLa and SiHa cells, a Western blot revealed the relative amounts of AKT and mTOR protein (, ). (g) Western blot revealing the relative EMT protein levels after overexpression and knockdown of ARTN in HeLa and SiHa cells (, , ).

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