Histone Deacetylase Inhibitor I3 Induces the Differentiation of Acute Myeloid Leukemia Cells with t (8; 21) or MLL Gene Translocation and Leukemic Stem-Like Cells

Figure 2

I3 induced minimal apoptosis in Kasumi-1, KG-1, MOLM-13, and THP-1 cells. (a) Cells were treated with I3 or SAHA for 72 h and cell apoptosis was determined by flow cytometric analysis. (b) Graph bars show the percentage of living cells and cells undergoing necrosis/apoptosis. Kasumi-1 cells were treated with 0.125, 0.25, and 0.5 μM of I3 or 0.5 μM of SAHA. KG-1 cells were treated with 0.6, 1.2, and 2.4 μM of I3 or 1 μM of SAHA. MOLM-13 cells were incubated with 0.45, 0.9, and 1.8 μM of I3 or 0.9 μM of SAHA. THP-1 cells were treated with 0.6, 1.2, and 2.4 μM of I3 or 1.2 μM SAHA (, ).

(a)

(b)