Long Noncoding RNA H19 Derived from M2 Tumor-Associated Macrophages Promotes Bladder Cell Autophagy via Stabilizing ULK1

Figure 1

M2-TAM-derived exosomes promote autophagy in bladder cancer cells. (a) Representative electron micrograph of exosomes derived from TAMs (scale bar, 100 nm). (b) The diameter of exosomes derived from the medium of M0 or TAMs. (c) Western blot analysis of the expression of CD9, CD81, and HSP70 in the cells and exosomes. (d) Western blot analysis of protein levels of p62 and LC3 in M0-exo or TAMs-exo-treated BC cells after starvation for 2 h. (e) Western blot analysis of protein levels of LC3 in M0-exo or TAMs-exo-treated BC cells with or without the presence of Baf A1 (0.1 μM) after starvation for 2 h. (f) Fluorescence image of GFP-LC3 in M0-exo or TAMs-exo-treated BC cells after starvation for 2 h. (g) Quantification of LC3-positive cells in (c). Data shown are representative images or expressed as the mean ± SD. compared to the control.

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