Long Noncoding RNA H19 Derived from M2 Tumor-Associated Macrophages Promotes Bladder Cell Autophagy via Stabilizing ULK1

Figure 4

Overexpression of H19 in TAMs-exo promotes autophagy in bladder cancer cells. (a) The efficiency of H19 overexpression was examined by qRT-PCR in TAMs exosomes. (b) Western blot analysis of protein levels of p62 and LC3 in control or H19-overexpressed TAMs-exosome-treated BC cells after starvation for 2 h. (c) Western blot analysis of protein levels of p62 and LC3 in control or H19-overexpressed TAMs-exosome-treated BC cells with or without the presence of Baf A1 (0.1 μM) after starvation for 2 h. (d) Fluorescence image of GFP-LC3 in control or H19-overexpressed TAMs-exosome-treated BC cells after starvation for 2 h. (e) Quantification of LC3-positive cells in (d). Data shown are representative images or expressed as the mean ± SD. compared to the control.

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