Long Noncoding RNA H19 Derived from M2 Tumor-Associated Macrophages Promotes Bladder Cell Autophagy via Stabilizing ULK1

Figure 5

TAMs-exo-derived lncRNA H19 stabilized ULK1 in bladder cancer cells. (a) Western blot analysis of protein levels of ULK1, ATG13, and ATG5 in control or H19-silenced TAMs-exosome-treated BC cells after starvation for 2 h. (b) Western blot analysis of protein levels of ULK1, ATG13, and ATG5 in control or H19-overexpressed TAMs-exosome-treated BC cells after starvation for 2 h. (c) After treatment with the protein synthesis inhibitor CHX for indicated times, the protein levels of ULK1 in H19-silenced TAMs-exosome-treated T24 cells were examined by western blot. (d) Quantification of ULK1 expression in (c). (e) Western blot analysis of K48-ub levels of ULK1 in control or H19-silenced TAMs-exosome-treated BC cells after starvation for 2 h. Data shown are representative images or expressed as the mean ± SD. compared to the control.

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