Prognostic Lnc-S100B-2 Affects Cell Apoptosis and Microenvironment of Colorectal Cancer through MLLT10 Signaling

Figure 6

Effects of Lnc-S100B-2 and MLLT10 on the tumor microenvironment. (a) The expressions of CD3 were detected by IF assay. (b) The percent of CD3 (-) CD16 (+) cells was detected by flow cytometry. (c) The percent of CD11b (+) cells was analyzed by flow cytometry. (d, e) The expression E-cadherin and vimentin was analyzed by IF assay. (f–l) The expressions of E-cadherin, N-cadherin, vimentin, β-catenin, snail, and slug were detected by qRT-PCR and Western blot. ^∗ < 0.05 versus NC group, ^# < 0.05 versus sh-MLLT10 group, ^& < 0.05 versus sh-Lnc-S100B-2 group, and one-way ANOVA.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)