Pancreatic Cancer-Derived Exosomes Promote the Proliferation, Invasion, and Metastasis of Pancreatic Cancer by the miR-3960/TFAP2A Axis

Figure 1

Screening of microRNAs of cancer-derived exosomes in pancreatic cancer based on bioinformatics and quantitative real-time PCR (qRT-PCR) analysis. () (a) The bar graph shows the top 30 differentially expressed genes of GSE50632 on the GPL16294 platform using GEO2R for analysis. The Log₂ (FC) of miR-3960 is 8.90065. (b) The Venn diagram presents the intersection of mRNAs retrieved by different databases for miR-3960. (c) The expression levels of PEG10, HOXB8, EN1, NRARP, PCDHA4, TFAP2A, PCDHA6, EGR3, PCDHA2, and PCDHA11 mRNA in PC tumor and normal groups were analyzed by the TCGA normal and GTEx data on the website of GEPIA. Red indicates the tumor group; grey indicates the normal group. (d) The miR-3960 level in HPDE6-C7, PANC-1, BxPC-3 and MIA cell lines by qRT-PCR analysis (n =3). (e-n) The effect of miR-3960 on the levels of PEG10, HOXB8, EN1, NRARP, PCDHA4, TFAP2A, PCDHA6, EGR3, PCDHA2, and PCDHA11 mRNA by qRT-PCR (n =3). ^★P <0.01 compared to the normal group; ^▲▲P <0.01 compared to the HPDE6-C7 group; P <0.05, P <0.01 compared to the miR-NC group.

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