SHC1 Promotes Lung Cancer Metastasis by Interacting with EGFR

Figure 2

Knockdown of SHC1 inhibits the proliferation, migration, and invasion of lung cancer cells and induces apoptosis. (a) SHC1 expression is upregulated in lung cancer cell lines. (b) Using qRT-PCR to detect the expression changes of SHC1 in A549 and H446 transfected with shRNA and SHC1 cells. (c) CCK-8 analysis showed that downregulation of SHC1 inhibited DNA synthesis in A549 and H446 cells. (d) The transwell experiment, downregulation of SHC1 inhibited the invasion ability of A549 and H446 cells. (e) The TUNEL test results show that knocking down SHC1 promotes the apoptosis of A549 and H446 cells. (f) qRT-PCR detects the expression of Bax in A549 and H446 cells after knocking down SHC1. (g) qRT-PCR detects the expression of Bcl-2 in A549 and H446 cells after knocking down SHC1. Data are shown as the mean ± SEM; n = 3 independent experiments, .

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