ANGPTL4 Regulates Lung Adenocarcinoma Pyroptosis and Apoptosis via NLRP3\ASC\Caspase 8 Signaling Pathway to Promote Resistance to Gefitinib

Figure 2

Knockdown with ANGPTL4 expression reduces the resistance of PC9/GR to gefitinib (, n = 3). (a, b) The CCK-8 test revealed the cell viability and IC₅₀ of gefitinib in A549, PC9, H1975, and BEAS-2B cells. (c, d) ANGPTL4 protein and mRNA expression in PC9/GR cells after transfection with ANGPTL4-shRNA and NC-shRNA. (e, f) CCK-8 test revealed the cell viability and IC₅₀ of gefitinib in PC9/GR cells after ANGPTL4 knockdown. Control: blank control group; NC-shRNA: PC9/GR cells transfected with NC-shRNA; G: PC9/GR cells + gefitinib; ANGPTL4-shRNA: PC9/GR cells transfected with ANGPTL4-shRNA; ANGPTL4-shRNA + G: PC9/GR cells transfected with ANGPTL4-shRNA + gefitinib. , , and : p < 0.05, 0.01, and 0.001, respectively, compared to the control group; ^#, ^## and ^###: p < 0.05, 0.01, and 0.001, respectively, compared to the PC9 group.

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