ANGPTL4 Regulates Lung Adenocarcinoma Pyroptosis and Apoptosis via NLRP3\ASC\Caspase 8 Signaling Pathway to Promote Resistance to Gefitinib

Figure 3

Knockdown with ANGPTL4 expression reduces the resistance of PC9/GR to gefitinib (, n = 3). (a) Transwell test revealed the correlation between ANGPTL4 knockdown and PC9/GR cell migration ability (×400). (b) Scratching experiments revealed a correlation between ANGPTL4 knockdown and the migration ability of PC9/GR cells (×200). (c) EdU proliferation test revealed the correlation between ANGPTL4 knockdown and PC9/GR cell proliferation (×400). (d) Flow cytometry showed the correlation between ANGPTL4 knockdown and PC9/GR cell apoptosis. Control: blank control group; NC-shRNA: PC9/GR cells transfected with NC-shRNA;G: PC9/GR cells + gefitinib; ANGPTL4-shRNA: PC9/GR cells transfected with ANGPTL4-shRNA; ANGPTL4-shRNA + G: PC9/GR cells transfected with ANGPTL4-shRNA + gefitinib. , , and : p < 0.05, 0.01, and 0.001, respectively, compared to the control group; ^# and ^##: p < 0.05 and 0.01, respectively, compared to the G group; ^&, ^&&, and ^&&&: p < 0.05, 0.01, and 0.001, respectively, compared to the ANGPTL4-shRNA group.

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