ANGPTL4 Regulates Lung Adenocarcinoma Pyroptosis and Apoptosis via NLRP3\ASC\Caspase 8 Signaling Pathway to Promote Resistance to Gefitinib

Figure 6

Changes in the expression of NLRP3\ASC\Caspase 8 pathway-related proteins after ANGPTL4 knockdown and overexpression in cells and transplanted tumour tissues (, n = 3). (a) NLRPS\ASC\Caspase 8 and pyroptosis-related protein expression after ANGPTL4 knockdown in PC9/GR cells. (b) Changes in the expression of NLRP3\ASC\Caspase 8 and apoptosis-related protein expression after ANGPTL4 knockdown in PC9/GR cells. (c) NLRP3\ASC\Caspase 8 and pyroptosis-related protein expression after ANGPTL4 knockdown in xenograft tumours. (d) NLRP3\ASC\Caspase 8 and apoptosis-related protein expression after ANGPTL4 knockdown in xenograft tumours. Control: PC9/GR cells; NC-shRNA: PC9/GR cells transfected with NC-shRNA;G: PC9/GR cells + gefitinib; ANGPTL4-shRNA: PC9/GR cells transfected with ANGPTL4-shRNA;ANGPTL4-shRNA + G: PC9/GR cells transfected with ANGPTL4-shRNA + gefitinib; Control + G: nude mice treated with gefitinib; NC-shRNA + G: nude mice treated with NC-shRNA cells + gefitinib; ANGPTL4-shRNA: nude mice treated with PC9/GR cells afterknockdown with ANGPTL4; ANGPTL4-shRNA + G: after knockdown with ANGPTL4 nude mice treated with PC9/GR cells + gefitinib. , and : p < 0.05,0.01 and 0.001, respectively, compared to the control or control + G group.

(a)

(b)

(c)

(d)