The Pyroptosis-Related Risk Genes APOBEC3D, TNFRSF14, and RAC2 Were Used to Evaluate Prognosis and as Tumor Suppressor Genes in Breast Cancer

Figure 10

The biological functions of APOBEC3D, TNFRSF14, and RAC2 in BC cells. (a-c) PCR results showed that the model gene expression was lower in MCF-7 and MDA-MB-231 cells. (d-f) Western blotting experiments showed that the APOBEC3D, TNFRSF14, and RAC2 protein expression were weaker in MCF-7 cells. Results of colony formation (g-i) and EdU (j-l) assays revealed that overexpression of APOBEC3D, TNFRSF14, and RAC2 greatly suppressed MCF-7 cell proliferation.

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