CircLATS2 Regulates miR-520a-3p/E2F7/p-VEGFR2 Signaling Pathway to Promote Hepatocellular Carcinoma Progression and Angiogenesis

Figure 3

miR-520a-3p targets E2F7. (a) Binding sites of miR-520a-3p and E2F7. The binding of miR-520a-3p to E2F7 was determined by dual luciferase reporter gene assay (b) and RNA pull-down (c). (d) qRT-PCR was used to detect the transfection efficiency of miR-520a-3p mimics in PLC-PRF-5 and HepG2 cells. (e) The effect of miR-520a-3p on E2F7 expression was detected by qRT-PCR. (f) qRT-PCR was used to detect the transfection efficiency of circLATS2. (g). The effect of circLATS2 on E2F7 expression was detected by qRT-PCR. (h) qRT-PCR was used to detect the expression level of E2F7 in paracancer and HCC tissues. HCC: hepatocellular carcinoma; qRT-PCR: quantitative real-time polymerase chain reaction.

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