Acidic Tumor Microenvironment Promotes Pancreatic Cancer through miR-451a/MEF2D Axis

Figure 5

Knockdown of MEF2D enhances MMP loss and ROS generation and inhibits stemness of PANC-1 cells. PANC-1 cells were transfected with sh-NC and sh-MEF2D for 48 h. (a) The oxygen consumption rate. (b) JC-1 staining was used to detect MMP loss of PANC-1 cells. (c) DCFH staining was used to detect ROS generation of PANC-1 cells. (d) The cell viability detected by CCK-8 assay. (e) Sphere formation assay was performed. (f) The expression of CD24, CD44, and ESA. Error bars, mean ± SD from three independent experiments.  < 0.05,  < 0.01, and  < 0.001.

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