Circular RNA hsa_circ_0004543 Aggravates Cervical Cancer Development by Sponging MicroRNA hsa-miR-217 to Upregulate Hypoxia-Inducible Factor

Figure 3

hsa_circ_0004543 was identified to serve as a sponge for hsa-miR-217 in CC cells. (a) Diagram of potential binding sites between hsa-miR-217 and hsa_circ_0004543 (https://circinteractome.nia.nih.gov/) with mutation sites for specific binding assay. (b) Dual-luciferase reporter activity of SiHa and C-4I cells cotransfected by hsa_circ_0004543 3′UTR wild-type or mutated reporter with or without hsa-miR-217 mimics. (c) hsa-miR-217 was pulled down and enriched with biotin-labeled hsa-miR-217 specific probe in CC cell lysates. (d) hsa-miR-217 expression in human CC cells (SiHa, CaSki, C-4I, C-33A, SW756, and HeLa) was significantly downregulated compared with normal human cervical epithelial cells (End1/E6E7). (e) hsa-miR-217 expressions in paired CC and paracancerous (NC) tissues from 40 local CC patients detected by qRT-PCR. (f) hsa-miR-217 expressions in hsa_circ_0004543 silenced SiHa and C-4I cells determined with qRT-PCR. (g) Pearson’s correlation analysis of associations between hsa-miR-217 and hsa_circ_0004543 expressions in CC patient tissues (n = 40) ( < 0.05,  < 0.01, and  < 0.001).

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