Circular RNA hsa_circ_0004543 Aggravates Cervical Cancer Development by Sponging MicroRNA hsa-miR-217 to Upregulate Hypoxia-Inducible Factor

Figure 5

hsa-miR-217 knockdown or HIF-1a overexpression rescued hsa_circ_0004543 silencing inhibited malignant phenotypes in CC cells. (a) Viability of SiHa and C-4I cells after hsa_circ_0004543 silencing with/without inh-hsa-miR-217 or HIF-1a overexpression determined using the CCK-8 assay. (b) Colony proliferation abilities of SiHa and C-4I cells after hsa_circ_0004543 silencing with/without inh-hsa-miR-217 or HIF-1α overexpression determined using colony formation assay. (c) Cell migration capability of SiHa and C-4I cells after hsa_circ_0004543 knockdown with/without inh-hsa-miR-217 or HIF-1a overexpression detected by transwell assay without Matrigel. (d) Cell invasion capability of SiHa and C-4I cells after hsa_circ_0004543 silencing with/without inh-hsa-miR-217 or HIF-1α overexpression detected using transwell assay with Matrigel ( < 0.05;  < 0.01, and  < 0.001).

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