A Novel circRNA hsa_circRNA_002178 as a Diagnostic Marker in Hepatocellular Carcinoma Enhances Cell Proliferation, Invasion, and Tumor Growth by Stabilizing SRSF1 Expression

Figure 5

Knockdown of SRSF1 antagonized the promotive effects of hsa_circRNA_002178 upregulation on HCC cell proliferation, invasion, and migration. pcDNA-hsa_circRNA_002178 was transfected alone or together with SRSF1 shRNA into Huh7 cells. (a-b) The efficiency of SRSF1 shRNA in Huh7 cells was tested by Western blotting. (c) The cell proliferation was tested by CCK-8 assay. (d-g)The cell invasion and migration abilities of Huh7 cells after transfection with pcDNA-hsa_circRNA_002178 or/and SRSF1 shRNA were detected by Transwell or damage repair assays. (h-i) The E-cadherin and Vimentin protein levels in Huh7 cells after transfection with pcDNA-hsa_circRNA_002178 or/and SRSF1 shRNA were employed by Western blotting. N = 3. .

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