EGFR-AS1 Promotes Nonsmall Cell Lung Cancer (NSCLC) Progression via Downregulating the miR-524-5p/DRAM1 Axis and Inhibiting Autophagic Lysosomal Degradation

Figure 1

EGFR-AS1 is vital for NSCLC pathogenesis. (a) The relative expression level of EGFR-AS1 in normal BEAS-2B cells and NSCLC cell lines. One-way ANOVA was applied. (b) The expression levels of EGFR-AS1 in 80 samples of normal lung tissues and NSCLC tissues. The paired-student t test was applied. (c) Cytoplasmic and nuclear expression of EGFR-AS1 in NSCLC cells was detected by RT-qPCR. (d) The knockdown effect of si-EGFR-AS1 in NSCLC cells was assessed by RT-qPCR. The unpaired-student t test was applied. (e) The effect of EGFR-AS1 knockdown on NSCLC cells’ viability. Two-way ANOVA was applied. (f) The effect of EGFR-AS1 knockdown on the NSCLC cells’ invasion. The unpaired-student t test was applied. (g) The effect of EGFR-AS1 knockdown on the tumor weight and volume in nude mice bearing HCC827 cells. Two-way ANOVA and the unpaired-student t test were applied for difference comparison in the line chart and bar chart, respectively. The data are represented as mean ± S.D.  < 0.01 and  < 0.001.

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