EGFR-AS1 Promotes Nonsmall Cell Lung Cancer (NSCLC) Progression via Downregulating the miR-524-5p/DRAM1 Axis and Inhibiting Autophagic Lysosomal Degradation

Figure 2

miR-524-5p is negatively correlated to EGFR-AS1. (a) In silico prediction of interaction between EGFR-AS1 and miR-524-5p. (b) Comparison of the different relative expressions of EGFR-AS1 and miR-524-5p enriched in Ago2 in NSCLC cells. The unpaired-student t test was applied. (c) The binding of miR-524-5p to EGFR-AS1 in NSCLC cells was assessed by luciferase reporter assay. The unpaired-student t test was applied. (d) The expression level of miR-524-5p in normal lung cells and NSCLC cells. One-way ANOVA was applied. (e) The expression level of miR-524-5p in 50 samples of normal lung tissues and NSCLC tissues. The paired-student t test was applied. (f) A negative correlation was found between miR-524-5p and EGFR-AS1 in NSCLC samples. Spearman’s correlation analysis was conducted. The data are represented as mean ± S.D.  < 0.05,  < 0.01, and  < 0.001.

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