EGFR-AS1 Promotes Nonsmall Cell Lung Cancer (NSCLC) Progression via Downregulating the miR-524-5p/DRAM1 Axis and Inhibiting Autophagic Lysosomal Degradation

Figure 5

The miR-524-5p/DRAM1 axis is vital for lung cancer cell biological processes. (a) Western blotting analysis was utilized to determine the effect of miR-524-5p inhibition on DRAM1 protein expression in NSCLC cells. One-way ANOVA was applied. (b) Western blot analysis was utilized to determine the effect of miR-524-5p inhibition on autophagy-related protein expression in NSCLC cells. One-way ANOVA was applied. (c) The CCK-8 assay was used to determine the effect of miR-524-5p inhibition and DRAM1 knockdown on NSCLC cell viability. Two-way ANOVA was applied. (d) The effect of inhibiting miR-524-5p and DRAM1 on NSCLC cell invasion. One-way ANOVA was applied. The data are represented as mean ± S.D.  < 0.01.

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