Long Noncoding RNA XIST Promotes Resistance to Lenvatinib in Hepatocellular Carcinoma Cells via Epigenetic Inhibition of NOD2

Figure 5

Silencing of NOD2 abrogates the effects of inhibition of lncXIST on sensitivity to lenvatinib in HCC cells. (a) HepG2/R cells were transfected with siRNAs as indicated for 24 h, and mRNA levels of NOD2 were measured. (b) HepG2/R cells were transfected with siRNAs as indicated for 24 h, and protein levels of NOD2 were measured. (c) HepG2/R cells were transfected with siRNAs as indicated for 24 h, then cells were treated with or without lenvatinib (10 μM) for 24 h, and cellular viabilities were measured. (d) HepG2 cells were treated as described above, and cellular death was measured. (f) HepG2 and Huh7 cells were transfected as indicated for 24 h, and indicated proteins were measured by western blots. (g) mRNA levels of NOD2 were measured. (h) HepG2 and Huh7 cells were transfected as indicated for 24 h, then cells were treated with or without lenvatinib for another 24 h, and cellular viabilities were measured. (i) HepG2 and Huh7 cells were transfected as indicated for 24 h, then cells were treated with or without lenvatinib for another 24 h, and cellular death was measured. (j) Caspase activities were measured. The data was presented as ; and .

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